Sample Essay on Characterization of Bacterial Biofilms for Wastewater Treatment



The issue of the reuse of wastewater as a sustainable water resource in towns is continuously growing. Research initially carried out at the division of Environmental Microbiology resulted in the separation of the probable bacterial key organisms with some of the efficient nutrient removal properties including Comamonas denitrificans, Brachymonas denitrificans and Aeromonas hydrophila. Effectiveutilization of these organisms for enhanced nutrient elimination in wastewater management applications necessitates the retention of the strains, for the purpose of proliferating and maintenance of the organic activity within the process. This can be attained through immobilizing the organisms by the use of suitable systems such as the biofilm development which was observed to provide very good results with respect to cell retention, proliferation and microbial activity.


The main aim of the study was to identify and characterize a suitable immobilization method with maintained biological activity for increased preservation of the chosen wastewater treatment chief organisms.


 The results from the investigation of the dynamics and characteristics of the nutrient removal activity and the effect of bacterial interaction indicated that all the assessed bacterial strains were capable of forming single strain biofilm provided a suitable nutrient supply is given. For the biofilm development, the formation of extracellular polymeric substances was discovered to be critical and the polysaccharide remains composition was discovered to vary depending on the bacterial strain, culture state and the existence of the biofilm. During the biofilm development, Dentrification and phosphorus elimination activity of the chief organism was preserved.


During the research, the intricacy of both the single and the mixed biofilm development and there reactions on biofilm performance were noted. Relevant data was generated which could be helpful for future process development as well as experimental design. This study is also underlined as important for the general understanding of biofilm microbiology.